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KMID : 0613820140240121339
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Journal of Life Science
2014 Volume.24 No. 12 p.1339 ~ p.1344
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Tissue Distribution of HuR Protein in Crohn’s Disease and IBD Experimental Model
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Choi Hye-Jin
Park Jae-Hong
Park Ji-Yeon
Kim Ju-Il
Park Seong-Hwan
Oh Chang-Gyu
Do Kee-Hun
Song Bo-Kyung
Lee Seung-Joon
Moon Yu-Seok
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Abstract
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Inflammatory bowel disease is an immune disorder associated with chronic mucosal inflammation and severe ulceration in the gastrointestinal tract. Antibodies against proinflammatory cytokines, including TNFα, are currently used as promising therapeutic agents against the disease. Stabilization of the transcript is a crucial post-transcriptional process in the expression of proinflammatory cytokines. In the present study, we assessed the expression and histological distribution of the HuR protein, an important transcript stabilizer, in tissues from experimental animals and patients with Crohn’s disease. The total and cytosolic levels of the HuR protein were enhanced in the intestinal epithelia from dextran sodium sulfate (DSS)-treated mice compared to those in control tissues from normal mice. Moreover, the expression of HuR was very high only in the mucosal and glandular epithelium, and the relative localization of the protein was sequestered in the lower parts of the villus during the DSS insult. The expression of HuR was significantly higher in mucosal lesions than in normal-looking areas. Consistent with the data from the animal model, the expression of HuR was confined to the mucosal and glandular epithelium. These results suggest that HuR may contribute to the post-transcriptional regulation of proinflammatory genes during early mucosal insults. More mechanistic investigations are warranted to determine the potential use of HuR as a predictive biomarker or a promising target against IBD.
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KEYWORD
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IBD (Inflammatory bowel disease), HuR protein, intestinal inflammation
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